INTRODUCTION TO INFECTION CONTROL IN CLINICAL SETTING
Body Defenses Against Infection: Understanding the Immune System in Nursing
“Immune System Defenses: Protecting the Body from Infection”

Explore the body’s natural defenses against infection and how the immune system functions to protect health. Essential nursing knowledge.
Table of Contents
Body Defenses Against Infection: Understanding the Immune System
The body’s defense against infections is a complex process. It involves the immune system. The system works to resist the transmission of harmful agents. Immunity is the body’s ability to recognize and eliminate foreign materials that enter the system. This process helps prevent infections, diseases, and other unwanted biological invasions.
What is Immunity?
Immunity is the ability to protect the body from pathogens such as bacteria, viruses, fungi, and other microorganisms. The immune system detects and fights off these harmful agents through the recognition of antigens. Antigens are proteins found on the surface of cells, viruses, fungi, bacteria, and even toxins or drugs. The immune system identifies these foreign substances and initiates responses to destroy or neutralize them.
Role of the Immune Response
The immune response refers to the body’s method of identifying and attacking harmful microorganisms. When the immune system is compromised or unable to respond properly, an infection may develop. Efficient immune responses are crucial in preventing the spread of diseases and maintaining overall health.
Inflammatory Response: The Body’s Defense Mechanism
Inflammation is a key part of the immune system’s response to infection, injury, and irritation. The body’s white blood cells release chemicals. This protects against harmful substances like bacteria, viruses, and chemicals. Inflammation results in redness, swelling, heat, and sometimes pain, all signs of the immune system working to fight off infection.
Types of Inflammation
- Acute Inflammation: Acute inflammation occurs immediately after an injury or infection, serving as a protective measure. It usually lasts for less than two weeks. Once the harmful agent is removed, the inflammation subsides, allowing healing and return to normal function.
- Chronic Inflammation: Chronic inflammation develops when the harmful agent remains or the inflammation process does not resolve. It may lead to tissue damage, scarring, and the persistence of infection. Chronic inflammation is linked to conditions like rheumatoid arthritis, autoimmune diseases, and cardiovascular diseases.
- Subacute Inflammation: This type of inflammation is a mix of acute and chronic inflammation. It may last longer than acute inflammation but does not fully develop into chronic inflammation. It is a less common stage and involves both tissue repair and ongoing immune responses.
Causes of Inflammation
Inflammation is a protective response by living tissue to any injury or harmful stimuli. It plays a crucial role in healing but can also cause damage if not properly controlled. The body releases chemicals from white blood cells to address infection, injury, or foreign substances. This leads to increased blood flow, swelling, and often pain at the site of injury.
- Physical Causes
- Mechanical injuries like car accidents, assaults, or falls.
- Environmental factors such as extreme cold or heat, including burns or frostbite.
- Chemical Causes
- Exposure to harmful chemicals such as acid burns, drugs, or venom from insects or animals.
- Infection
- Bacteria, viruses, fungi, or parasites can invade the body, causing infection and triggering an inflammatory response.
- Ischemia
- Restricted blood supply to tissues, often due to a blockage or injury, leading to tissue damage and even cell death.
- Immune Responses
- Autoimmune diseases (e.g., rheumatoid arthritis) and allergies can cause the immune system to attack the body’s own tissues, leading to chronic inflammation.
Signs of Inflammation: Identifying the Response
Inflammation manifests in 5 cardinal signs, each indicating a physiological reaction to tissue injury:

- Redness (Erythema):
- Caused by the dilation (widening) of blood vessels, leading to increased blood flow to the affected area.
- Warmth (Heat):
- Due to increased blood flow and metabolic activity in the area of inflammation.
- Swelling (Edema):
- Results from fluid and white blood cells (leukocytes) accumulating in the tissues, causing the area to expand.
- Pain:
- Triggered by the release of chemicals from damaged cells, and may also involve the irritation of nerves in the area.
- Loss of Function:
- Caused by increased swelling and pain, which may impair movement or proper functioning of the affected area.
Additionally, fever may occur as a result of the release of endogenous pyrogens from immune cells like neutrophils and macrophages.
The Process of Inflammation: How the Body Responds
The process of inflammation involves a series of steps designed to protect and heal tissues. Here’s how it works:
- Vasodilation:
- The arterioles (small blood vessels) near the injury site widen, increasing blood flow, leading to redness and warmth.
- Vascular Permeability:
- Endothelial cells in blood vessels become more permeable. This permeability allows fluid, white blood cells, and chemical mediators to leak into the surrounding tissues.
- Exudation:
- Fluid, proteins, red blood cells, and white blood cells move from the bloodstream into the tissue. They help to fight infection. They also aid tissue repair.
- Vascular Stasis:
- Blood flow slows down. This allows chemical mediators and immune cells to gather and focus on healing. They also work on defending the affected tissue.
Stages of the Inflammatory Reaction
The inflammatory reaction is a complex and overlapping sequence of events that occur in response to tissue injury. Although these events happen simultaneously, they can be grouped into distinct stages:
1. Tissue Injury
Tissue damage occurs from various causes, including trauma (e.g., tackles, collisions, falls) or microtrauma (repetitive strain or overuse). This injury triggers the body’s inflammatory response, which is essential for the healing process.
2. Release of Chemicals
When tissue cells are injured, they release a variety of chemical mediators, including:
- Histamine
- Serotonin
- Bradykinin
- Prostaglandins
- Lymphokines
These chemicals initiate the inflammatory process by causing vasodilation (widening of blood vessels). They also increase vascular permeability (leakage of fluid from capillaries). The increased blood flow to the affected site causes the classic signs of redness (erythema) and warmth. The chemical mediators act as messengers. They attract white blood cells (leukocytes) to the area. This process is known as chemotaxis.
3. Leukocyte Migration
In response to chemotaxis, leukocytes (white blood cells) migrate to the injured area. Two main types of leukocytes involved in this phase are:
- Neutrophils: These are the first responders to the injury. They help neutralize harmful bacteria and clear debris from the site.
- Macrophages: These cells arrive 72 hours after injury and continue to clear debris and bacteria. They play a crucial role in the healing process, helping to prepare the area for new tissue growth.
These cells engulf bacteria, dead cells, and other debris. This engulfing is essential for clearing the area. It allows new cells to regenerate. Over time, destroyed tissue is replaced by fibrous (scar) tissue, which can affect the tissue’s functional capacity.
4. Tissue Healing
Tissue healing occurs in several overlapping stages:
- Collagenation:
Fibroblasts start producing collagen, creating a matrix that supports new tissue growth. This process occurs as macrophages clean the injured area and prepare it for regeneration. - Angiogenesis:
New blood vessels (capillaries) form in a process called angiogenesis or revascularization. This provides the necessary blood supply to the healing tissue, facilitating the growth of new cells. For instance, in muscle injuries, muscle cells repopulate the area as blood flow is restored.
5. Proliferation
This phase can last up to 4 weeks. During this time, the body focuses on tissue repair. In cases of severe injury, the area may form granulation tissue. It consists of a mixture of specific tissue cells and other cells. If this tissue is not removed, it can turn into scar tissue, which may decrease the function of the tissue.
6. Remodeling
Remodeling is the final stage of healing. In this stage, the new cells and collagen fibers are reorganized. They adapt to the tissue stresses. This phase can take months or even years, as the tissue gradually regains its functionality. Proper stretching can optimize the strength of the new tissue during this phase. Rehabilitation helps improve its ability to function normally.
Immune Response
The immune response includes several defense mechanisms. The body uses these to protect itself from foreign invaders, such as bacteria, viruses, and other pathogens. It can be broken down into three lines of defense: physical and chemical barriers, nonspecific responses, and specific responses. These defenses work together to protect the body from infections.
A. Nonspecific Immune Responses
Nonspecific immune defenses are the body’s first line of defense against pathogens. They act as general defenses and are not tailored to specific types of pathogens. They include:
1. Physical and Chemical Barriers
- Skin: The skin serves as the body’s primary physical barrier. When intact, it prevents microorganisms from entering the body.
- Mucous Membranes: These membranes secrete mucus to trap microbes, preventing their spread into deeper tissues.
- Hair: Hair in the nose filters microbes, dust, and pollutants from the air, preventing them from entering the respiratory system.
- Cilia: The cilia in the upper respiratory tract trap inhaled debris. They move it toward the throat to be expelled from the body.
- Stomach Acid: The hydrochloric acid in the stomach kills pathogens that are ingested through food or water. This serves as a chemical barrier to infection.
- Lysozymes: Found in tears, sweat, and saliva. Lysozyme is an enzyme that breaks down bacterial cell walls. It acts as a natural antibiotic.
- Hyaluronic Acid: This gelatinous substance in tissues slows the spread of harmful microorganisms.
- Saliva: Saliva dilutes microorganisms in the mouth, washing away debris. It contains microbial inhibitors like lysozyme, lactoferrin, and secretory IgA.
- Sebum: Produced by the skin, sebum contains unsaturated fatty acids that create a protective film, inhibiting bacterial growth.
2. Inflammatory Response
When tissue is damaged due to injury or infection, the body activates the inflammatory response. This nonspecific reaction involves an increased blood supply to the affected area. There are changes in vascular permeability. This allows immune cells to reach the site of injury or infection. Key features of inflammation include:
- Swelling
- Redness
- Heat
- Pain
The inflammatory response ensures that immune cells, such as neutrophils and mononuclear cells, move to the site of infection. At this location, they combat invading pathogens.
B. Specific Immune Responses
When nonspecific defenses are not enough to eliminate an infection, the body activates the specific immune response. This response is part of acquired immunity. This response targets specific pathogens and involves various components, such as antibodies and T cells.
1. Phagocytic Immune Response
Phagocytosis is the process in which phagocytes (such as neutrophils and macrophages) engulf and digest microorganisms. Phagocytes also remove dead or dying cells from the body, maintaining tissue health. Apoptosis, a form of programmed cell death, helps eliminate potentially harmful cells in an orderly manner.
2. Antibody Immune Response
This component, also known as humoral immunity, involves B lymphocytes (B cells) that produce antibodies. Antibodies are large molecules that target specific pathogens. The antibody immune response is effective against:
- Extracellular bacterial pathogens
- Viruses that enter through the respiratory or intestinal tracts
- Prevention of recurrent viral infections
There are five major classes of immunoglobulins (antibodies): IgG, IgE, IgD, IgM, and IgA. These antibodies play crucial roles in recognizing and neutralizing pathogens.
3. Cellular Immune Response
Cell-mediated immunity does not involve antibodies. Instead, it relies on T lymphocytes (T cells). There are two main types of T cells:
- Cytotoxic T cells: These cells destroy infected or abnormal cells.
- Helper T cells: These cells assist other immune cells by releasing chemical signals called lymphokines.
When a T cell recognizes an antigen, it binds to it and becomes sensitized. Sensitized T cells travel to the infection site, where they release lymphokines to attract macrophages. This type of immunity is crucial for combating viral infections, slow-growing bacterial infections, fungal infections, and cancerous cells.
Stages of Immune Response
The immune response follows a structured process that can be divided into four distinct stages: recognition, proliferation, response, and effector. Each stage plays a critical role in defending the body from infections and ensuring a tailored, effective immune reaction.
(i) Recognition Stage
The first step in the immune response is for the body to recognize foreign invaders. The immune system must identify substances or pathogens that are not part of the body (non-self). This recognition process involves lymphocytes and macrophages:
- Lymph nodes and lymphocytes constantly survey the body through the bloodstream.
- Macrophages encounter foreign material, either removing or imprinting its structure to mark it as an invader.
- The immune system distinguishes between the body’s own structures (self) and foreign markers (antigens). This differentiation triggers the next stage of the immune response: proliferation.
(ii) Proliferation Stage
In the proliferation stage, the immune system amplifies the response:
- The lymphocyte that encountered the foreign invader returns to the lymph node to relay the message.
- This stimulates the activation of T lymphocytes and B lymphocytes, which enlarge, divide, and proliferate in response to the antigen.
- T lymphocytes, also known as cytotoxic T cells (killer T cells), are prepared to directly attack infected cells.
- B lymphocytes stimulate the production and release of antibodies, essential for neutralizing pathogens.
(iii) Response Stage
In this stage, the immune system’s response is carried out either in a humoral or cellular fashion:
- Humoral Response: The B lymphocytes produce antibodies in response to the antigen. These antibodies are released into the bloodstream and circulate in the plasma, targeting pathogens or toxins directly.
- Cellular Response: The sensitized T cells return to the lymph nodes. There, they stimulate other lymphocytes. They particularly stimulate cytotoxic T cells (killer T cells). These cells then attack and destroy the infected cells directly by binding to the pathogen or infected tissue.
(iv) Effector Stage
The final stage involves the effector action, where the immune system clears the infection:
- Antibodies or cytotoxic T cells bind to the antigens on the surface of the foreign invader.
- This interaction triggers a series of events that lead to the destruction of the pathogen or the neutralization of toxins.
- The complement system, along with killer T cells, helps amplify this process. It ensures that the invader is effectively eliminated from the body.
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INTRODUCTION TO INFECTION CONTROL IN CLINICAL SETTING
Biomedical Waste Management: Segregation, Disposal & Color Coding
Understand biomedical waste management, including hospital waste types, segregation, disposal, color coding, storage, transportation, and treatment for safer healthcare practices.

Learn about biomedical waste management, types of hospital waste, segregation, disposal methods, color coding, storage, and treatment of medical waste.
Table of Contents
Biomedical Waste Management
Definition of Biomedical Waste
Medical Waste: Any waste generated during the diagnosis, treatment, or immunization of humans or animals. This includes waste produced during research related to these activities. It also covers waste from the production or testing of biological materials.
Biomedical Waste: Waste produced during medical activities, including diagnosis, treatment, immunization, research, and biological production/testing. It also includes waste from slaughterhouses.
Biomedical waste is any waste contaminated with patients’ body fluids, including:
- Syringes & Needles
- Ampoules
- Organs & Body Parts
- Placenta & Dressings
- Disposable Plastics
- Microbiological Waste
Principles of Biomedical Waste Management (3Rs)
- Reduce: Minimize waste generation.
- Recycle: Reprocess materials when possible.
- Reuse: Safely reuse items where applicable.
Importance of Biomedical Waste Management
1. Environmental Protection
- Reduces air, water, and soil pollution.
- Supports government initiatives for a cleaner environment.
2. Prevention of Infections & Health Hazards
- Prevents the spread of infectious diseases like HIV, Hepatitis B, and Tuberculosis (TB).
- Protects healthcare workers, patients, and the community from hazardous exposure.
3. Legal Compliance
- The Biomedical Waste Management Rules (1996) make proper waste disposal a legal obligation.
- Hospitals must follow government guidelines to avoid penalties.
Types of Hospital Waste
1. Non-Hazardous Waste
- Includes general waste from kitchens, wards, laboratories, and offices.
- Example: Paper, wrappers, stationery, food waste.
2. Hazardous/Risk Waste
- Includes potentially infectious and toxic waste.
a) Potentially Infectious Waste
- Human anatomical waste: Tissues, organs, body parts.
- Items contaminated with body fluids: Blood, pus, laboratory samples.
- Sharps & Needles: Hypodermic needles, syringes, scalpels, razors, surgical instruments.
b) Potentially Toxic Waste
- Radioactive Waste: Solids, liquids containing radioactive materials.
- Chemical Waste: Toxic, corrosive, flammable substances.
- Pharmaceutical Waste: Expired or unused drugs.
Decontamination & Disposal of Hospital Waste
1. Puncturing/Mutilation
- Disposable items (gloves, syringes, IV bottles, catheters) must be punctured before disposal.
- Needles & Syringes should be cut using a syringe cutter.
2. Chemical Decontamination
- Bleach Solution: 10 gm bleach per 1 liter of water.
- Hypochlorite Solution: At least 1% concentration.
3. Deep Burial
- Used for biomedical waste that cannot be incinerated.
Dilution of Chlorine Releasing Compounds
Available Chlorine | Required Chlorine | Sodium Hypochlorite Solution (5%) | Calcium Hypochlorite (70%) |
---|---|---|---|
Clean Condition | 0.1% | 20 ml/Liter | 14 gm/Liter |
Dirty Condition | 0.5% | 100 ml/Liter | 7 gm/Liter |
Waste Segregation
What is Waste Segregation?
Waste segregation is the process of separating different types of waste at the source to reduce risks and handling costs. It helps prevent infections and ensures safe disposal.
Importance of Segregation:
- Separates infectious/toxic waste from non-infected/non-toxic waste.
- Reduces the chances of infection.
- Lowers treatment and disposal costs.
- Ensures proper biomedical waste management.
Key Guidelines for Biomedical Waste Segregation:
- Biomedical waste must not be mixed with other waste.
- It should be separated into labeled containers/bags at the point of generation.
- Segregation at the source prevents infections and injuries.
- Waste is categorized into different colored containers based on disposal methods.
Categories of Biomedical Waste & Disposal Methods:
Category | Waste Type | Disposal Method |
---|---|---|
1 | Human anatomical waste | Incineration, deep burial |
2 | Animal waste | Incineration, deep burial |
3 | Microbiology & Biotechnology waste | Autoclaving, microwaving, incineration |
4 | Waste sharps (needles, syringes, blades, etc.) | Chemical disinfection, autoclaving, shredding |
5 | Discarded medicines & cytotoxic drugs | Incineration, secured landfill |
6 | Soiled waste (cotton, dressings, blood-contaminated items) | Incineration, microwaving, autoclaving |
7 | Solid waste (tubing, catheters, IV sets) | Chemical disinfection, autoclaving, shredding |
8 | Liquid waste (from labs, cleaning, disinfection) | Chemical treatment, drain disposal |
9 | Chemical waste (disinfectants, insecticides) | Chemical treatment, secured landfill |
Biomedical Waste Color Coding:
Why is Color Coding Important?
- Helps in proper waste segregation at the source.
- Reduces risk of infection and contamination.
- Ensures safe disposal and treatment of waste.
Color Coding System for Biomedical Waste
Color Code | Type of Container | Waste Category | Treatment & Disposal |
---|---|---|---|
Yellow | Plastic bags | Human & animal waste, biological waste, solid waste (Cat. 1, 2, 3, 6) | Incineration, Deep burial |
Red | Disinfected container/plastic bags | Microbiological & soiled waste, solid waste (Cat. 3, 6, 7) | Autoclave, Microwave, Chemical treatment |
Blue/White/Transparent | Puncture-proof container/bags | Waste sharps, solid waste (Cat. 4, 7) | Autoclave, Microwave, Chemical treatment, Shredding |
Black | Plastic bags | Discarded medicines, cytotoxic drugs, incineration ash, chemical waste | Secured landfill disposal |
Green | Plastic container | General waste (office, food, garden waste) | Secured landfill disposal |
Key Points to Remember
- Yellow Bag: Used for highly infectious waste like anatomical waste, body parts, placentas, and blood-soaked items.
- Red Bag: For contaminated plastic waste like syringes, IV tubes, catheters, and gloves.
- Blue/White Bag: For sharp objects like needles, blades, and broken glass.
- Black Bag: For non-infectious dry waste and chemical waste.
- Green Bag: For general waste like food and office waste.

Storage, Transportation, and Treatment of Biomedical Waste
1. Storage of Biomedical Waste
- Definition: Holding biomedical waste temporarily before treatment and disposal.
- Storage Time Limit:
- Big hospitals: 8-10 hours
- Other healthcare institutions: Up to 24 hours
- Maximum limit: 48 hours (Beyond this, permission from authorities is required.)
- Storage Guidelines:
- Waste must be labeled with ward/room details for traceability.
- Storage areas should have caution signs to prevent mishandling.
2. Transportation of Biomedical Waste
- Within the Hospital:
- Use wheeled trolleys, containers, or carts exclusively for biomedical waste.
- Daily cleaning of transport equipment is mandatory.
- Offsite Transportation:
- Bags should be sealed properly to prevent leakage/spillage.
- Infectious/toxic waste must be transported separately from municipal waste.
- A signed document by a nurse/doctor should accompany the waste, noting:
- Date, Shift, Quantity, Destination
- Vehicles must:
- Display the biohazard symbol.
- Have name and address of the carrier.
- Be easily cleanable with rounded corners.
3. Treatment of Biomedical Waste
Treatment: A process that changes the physical, chemical, or biological characteristics of waste to make it non-hazardous.
Treatment Method | Process | Waste Type |
---|---|---|
Incineration | Controlled burning at 982°C – 1093°C | Infectious, pathological, cytotoxic waste |
Deep Burial | Waste is buried in a 2m deep pit, covered with lime & soil | Anatomical waste, microbiological waste |
Autoclaving | Steam sterilization under high pressure & temperature | Infectious plastic waste, microbiological waste |
Microwaving | Uses moist heat & steam to disinfect waste | Pathological & infectious waste |
Irradiation | Uses gamma radiation (Cobalt-60) to kill microbes | Sharps, microbiological waste |
Chemical Treatment | Disinfection with sodium hypochlorite, chlorine dioxide, hydrogen peroxide | Liquid waste, chemical waste, sharps |
Shredding & Destruction | Physically breaks down waste into small particles | Plastic waste, sharps |
Secured Landfill | Waste is buried safely to prevent environmental contamination | Discarded medicines, chemical waste |
Hazards of Health-Care Waste:
1. What is Health-Care Waste?
- It includes general waste and hazardous waste.
- If infectious waste mixes with general waste, all waste becomes infectious.
- Improper disposal can spread infections and increase harmful microbes.
2. Who is at Risk?
People exposed to hazardous health-care waste include:
- Health workers: Doctors, nurses, hospital staff, and maintenance workers.
- Support workers: Laundry staff, waste handlers, and transport workers.
- Patients and visitors: In hospitals or receiving home care.
- Waste disposal workers: Those working in landfills or incinerators.
3. Types of Hazardous Health-Care Waste
Type of Waste | Examples |
---|---|
Sharps | Needles, scalpels, blades |
Genotoxic Waste | Cytotoxic drugs, genotoxic chemicals |
High Heavy Metal Waste | Batteries, broken thermometers, BP gauges |
Chemical Waste | Lab reagents, disinfectants, solvents |
Pathological Waste | Body parts, blood, other fluids |
Pressurized Containers | Gas cylinders, aerosol cans |
Infectious Waste | Lab cultures, isolation ward waste, tissues |
Pharmaceutical Waste | Expired or unused medicines |
4. Health Hazards
- Infectious Waste & Sharps: Causes infections like skin, respiratory, and gastrointestinal diseases. Pathogens enter through cuts, inhalation, or ingestion.
- Chemical & Pharmaceutical Waste: Can cause poisoning, burns, and injuries from toxic, flammable, or reactive chemicals.
- Genotoxic Waste: Exposure may happen through inhalation, skin contact, or ingestion, leading to severe health effects.
- Radioactive Waste: Can cause dizziness, vomiting, and serious genetic damage due to radiation exposure.
5. Prevention & Safe Disposal
- Proper segregation: Keep infectious waste separate from general waste.
- Safe handling & storage: Use protective gear and follow disposal guidelines.
- Proper disposal: Use incineration, landfill, or chemical treatment as needed.
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INTRODUCTION TO INFECTION CONTROL IN CLINICAL SETTING
7 Essential Personal Protective Equipment (PPE): Donning, Doffing & Precautions
Personal Protective Equipment (PPE) is crucial for infection control. Learn about proper donning and doffing, gowning, gloving, and standard precautions to ensure safety in healthcare environments.

Learn about Personal Protective Equipment (PPE). Understand proper donning and doffing. Familiarize yourself with gowning and gloving. Follow standard precautions to ensure safety in healthcare settings.
Table of Contents
Personal Protective Equipment (PPE)
What is PPE?
Personal Protective Equipment (PPE) includes clothing and gear. Healthcare workers use them to protect themselves from infectious agents. These include bacteria and viruses. It acts as a barrier between the worker and harmful germs found in blood, body fluids, or contaminated surfaces.
Why is PPE Important?
✅ Prevents the spread of infections.
✅ Protects healthcare workers from exposure to germs.
✅ Reduces the risk of contamination.
Types of PPE
- Gloves – Protect hands from direct contact with blood, body fluids, and contaminated surfaces.
- Gowns/Coverall – Protects the body and clothing from splashes and spills.
- Masks & Respirators – Prevent inhalation of infectious droplets.
- Triple-layer mask: Protects from droplets.
- N95 mask: Filters airborne particles and provides a tight facial seal.
- Face Shield – Covers the face and prevents splashes into the eyes, nose, and mouth.
- Goggles – Protects only the eyes from splashes and sprays.
- Head Covers – Covers the head and hair to prevent contamination.
- Shoe Covers – Prevents germs from spreading through footwear.

Principles of Using PPE
✔ Wash hands before and after wearing PPE.
✔ Check PPE before use; replace if damaged.
✔ Use PPE properly to ensure full protection.
✔ Remove PPE immediately after patient contact.
✔ Never reuse disposable PPE.
✔ Store PPE safely to prevent contamination.
Donning and Doffing of Personal Protective Equipment (PPE)
Proper donning and doffing of personal protective equipment (PPE) is crucial to reducing the risk of exposure to pathogens. Effective use of PPE includes correctly wearing, removing, and disposing of contaminated equipment.
Donning PPE
PPE must be donned correctly and in the proper order before entering a patient care area. Modifications to PPE should not be made once inside the care area. Ensure you are hydrated and have used the restroom beforehand, as PPE may need to be worn for extended periods. Perform thorough hand hygiene before beginning.
Steps for Donning PPE:
- Remove personal items – Remove home clothes, jewelry, watch, etc., and wear a clean hospital scrub suit.
- Check PPE kit – Ensure all components are present: mask, gown, gloves, head cover, shoe cover, and face shield.
- Perform hand hygiene – Use hand sanitizer or wash hands with soap and water.
- Wear shoe covers – Sit comfortably and put on shoe covers.
- Put on the first pair of gloves – Ensure proper fit.
- Wear the gown. Make sure the sleeves cover the gloves. The gown should fully cover the body when tied or closed.
- Put on the mask/respirator –
- Hold the respirator in one hand and bring it to the face.
- Pull the lower elastic band overhead and position it below the ears.
- Pull the upper elastic band overhead and position it above the ears.
- Pinch the nose clip to ensure a tight fit and check for a snug seal by exhaling three times.
- Wear the face shield or goggles – Ensure full coverage of the face.
- Wear the second pair of gloves – Gloves should go over the cuff of the gown.
Doffing PPE
Removing used PPE is a high-risk process that must follow a structured procedure to minimize self-contamination. A trained observer or doffing assistant and a designated removal area may be necessary.
Steps for Doffing PPE:
- Sanitize gloves – Rub hands together while wearing gloves.
- Remove shoe covers – Touch only the outer surface.
- Remove the second pair of gloves – Dispose of them properly.
- Remove the gown –
- Grasp the front of the gown and slowly pull it away, breaking the ties at the back.
- Pull the gown away from the body, rolling it into a bundle with the contaminated surface inside.
- Discard it in the appropriate receptacle.
- Remove the face shield –
- Tilt the head forward, grasp the strap, and gently pull it overhead.
- Remove the shield away from the face.
- Remove goggles –
- Grasp the earpieces behind the ears and pull them away from the face, avoiding contact with the front surface.
- Remove the inner gloves – Perform hand hygiene and put on a new pair of sterile/unsterile gloves.
- Remove the mask/respirator –
- Gently pull the lower elastic band overhead, followed by the upper band, and remove the mask away from the face.
- Remove the last pair of gloves – Perform thorough hand hygiene.
Gowning
Definition:
Gowning is an essential procedure in maintaining a sterile environment during surgery. It prevents contamination and ensures patient safety.
General Guidelines:
- Only the scrub nurse should gown and glove herself.
- Other team members should be gowned and gloved as they enter the room.
- Once gowned and gloved, stay in the sterile area until the setup is complete.
- Hands should be kept above the waist and in front of the body.
- If the gown gets contaminated with blood or fluids, change it immediately.
Steps of Gowning:
- Pick up the gown – Hold it from the inside top layer without touching unsterile areas.
- Let the gown unfold – Step back and allow it to drop without touching anything.
- Insert arms into sleeves – Keep hands at shoulder level while sliding arms into the sleeves.
- Assistant helps fasten the gown – The assistant secures the gown at the back without touching the sterile side.
Removing the Gown:
- If disposable, remove gloves along with the gown and roll them together.
- If not breakaway, untie the waist first, then the neck.
- Pull the gown away from the shoulders with ungloved hands.
- Roll the gown into a bundle without touching the outer contaminated surface.
This ensures a sterile environment and prevents infection risks.
Gloving
Definition:
Gloves provide a protective barrier. They shield healthcare workers and patients from infectious microorganisms found in blood, body fluids, and waste. Double gloving (wearing two gloves) can further reduce contamination risk during major surgeries.
Steps for Putting on Surgical Gloves:
- Prepare the gloves – After scrubbing, ask someone to open the glove package.
- Pick up the first glove – Use the left hand to grasp the cuff of the right glove.
- Wear the right glove – Insert the right hand and pull the glove on, keeping the cuff turned down.
- Pick up the second glove. Use the fingers of the gloved right hand. Grasp the cuff of the left glove.
- Wear the left glove – Insert the left hand. Pull the glove on. Ensure the cuff covers the gown sleeve.
- Adjust the cuffs – Pull the cuffs properly over the gown sleeve to secure the gloves.
Steps for Removing Surgical Gloves:
- Loosen the first glove – Grasp near the cuff and pull it partway off, turning it inside out.
- Loosen the second glove – Keep the first glove partially on while removing the second one.
- Remove both gloves together – Carefully pull off both gloves at the same time, touching only the inside surfaces.
- Dispose of gloves properly – Avoid splashing contaminants and discard gloves immediately.
- Wash hands thoroughly – Always clean hands after glove removal.
Proper gloving and removal help maintain sterility and prevent infections.
Standard Precautions:
Definition:
Standard precautions are measures for infection control. They are used to prevent the spread of diseases through blood, body fluids, non-intact skin, and mucous membranes. These precautions apply to all patients, reducing infection risks for both healthcare workers and patients.
Where to Use Standard Precautions?
Standard precautions should be used in any setting where there is exposure to:
✔ Blood
✔ Body fluids (semen, vaginal secretions, amniotic fluid, cerebrospinal fluid, pleural fluid, etc.)
❌ Standard precautions are not required for feces, urine, vomit, sweat, saliva, nasal secretions, and sputum unless they contain visible blood.
Who Should Follow Standard Precautions?
✔ Doctors, nurses, and healthcare workers
✔ Support staff (housekeeping, laundry, etc.)
Standard Precaution Practices:
- Hand Hygiene – Wash hands with soap and water or use an alcohol-based hand rub to prevent infections.
- Personal Protective Equipment (PPE) – Wear gloves, masks, gowns, and goggles as needed to prevent exposure.
- Respiratory Hygiene – Cover mouth and nose while coughing or sneezing; use tissues and masks to prevent droplet infections.
- Sharps Safety – Dispose of needles and sharp objects properly to avoid injuries and transmission of bloodborne infections.
- Safe Injection Practices – Use sterile syringes and needles only once to prevent infections.
- Sterile Instruments and Devices – Clean, disinfect, and sterilize reusable medical instruments before use on another patient.
- Clean and Disinfected Environment – Regularly clean and disinfect patient areas and equipment to prevent the spread of microorganisms.
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INTRODUCTION TO INFECTION CONTROL IN CLINICAL SETTING
🔴 Nosocomial Infection & Asepsis: Hand Washing Tips for Nursing Students
Discover effective ways to prevent nosocomial infections through asepsis and proper hand washing. Essential for nursing students to ensure patient safety.

Learn nosocomial infection prevention, asepsis techniques, and essential hand washing tips for nursing students. Improve patient safety and hygiene.
Table of Contents
Health Care-Associated Infection (Nosocomial Infection)
What is Nosocomial Infection?
- Nosocomial infections are infections acquired in hospitals or healthcare settings.
- Also called Hospital-Acquired Infections (HAIs).
- Occurs 48 hours after hospital admission or within 30 days after discharge.
- Caused by antibiotic-resistant organisms.
Who is at Risk?
- All hospitalized patients, but higher risk for:
- Young children and elderly
- Patients with weak immune systems
- Long hospital stays
- Use of catheters and medical devices
- Poor hand hygiene by healthcare workers
- Overuse of antibiotics
Why are Nosocomial Infections Common?
- Hospitals have many sick people with weak immune systems.
- Frequent movement of staff between patients spreads infections.
- Some medical procedures bypass the body’s natural protection.
- Unclean hospital environments and equipment.
How are Nosocomial Infections Spread?
- Direct Contact (Patient to patient, Staff to patient, Patient to visitors)
- Indirect Contact (Contaminated medical instruments, Hospital equipment)
- Through Air (Aerosols)
- Through Vectors (Flies, insects)
Common Sites and Causes of Nosocomial Infections
1. Urinary Tract Infections
- Caused by catheter use, poor hand hygiene, contaminated equipment.
- Reflux of urine from the bag to the bladder.
2. Surgical Wound Infections
- Caused by improper sterilization, poor handwashing, unclean dressing materials.
3. Bloodstream Infections
- Due to contaminated IV fluids, needles, or improper care of insertion sites.
Prevention of Nosocomial Infections
- Hand Hygiene: Wash hands properly for at least 20 seconds with soap and water.
- Use of Protective Equipment: Wear gloves, gowns, masks, and face shields when necessary.
- Proper Cleaning and Disinfection:
- Clean hospital rooms and equipment with disinfectants regularly.
- Linens should be sanitized and changed daily.
- Infection Control Policies:
- Identify high-risk patients.
- Isolate contagious patients.
- Education and Training:
- Healthcare workers should receive infection control training regularly.
- Safe Medical Practices:
- Proper use and care of catheters and IV lines.
- Avoid overuse of antibiotics.
Asepsis
Asepsis is the absence of disease-causing microorganisms. It helps prevent infections, especially during medical procedures.
Types of Asepsis
- Medical Asepsis (Clean Technique)
- Reduces the number of microorganisms and prevents their spread.
- Examples: Handwashing, wearing gloves, cleaning surfaces.
- Surgical Asepsis (Sterile Technique)
- Eliminates all microorganisms, including spores.
- Used in surgeries and invasive procedures.
Aseptic Techniques
Aseptic techniques help prevent infections by maintaining cleanliness.
Aims of Aseptic Technique
- Prevent infections in patients.
- Stop the spread of microorganisms.
- Protect healthcare workers from infections.
Indications for Aseptic Technique
Used during procedures like:
- Wound care and suturing.
- Inserting catheters, IV lines, and tubes.
- Surgical procedures and biopsies.
Principles of Medical Asepsis
- Wash hands before and after patient contact.
- Wear gloves and masks when needed.
- Keep soiled items separate from clean ones.
- Dispose of waste properly to prevent contamination.
- Avoid touching face, nose, or mouth while handling sterile items.
Principles of Surgical Asepsis
- Only sterile objects can touch other sterile objects.
- Open sterile packages carefully to avoid contamination.
- Use sterile drapes to create a sterile field.
- Do not talk, cough, or sneeze over a sterile field.
- Never turn your back on a sterile field.
- Consider the outer 1 inch of a sterile field contaminated.
Differences Between Medical and Surgical Asepsis

Medical Asepsis (Clean) | Surgical Asepsis (Sterile) |
---|---|
Reduces microorganisms | Eliminates all microorganisms |
Prevents the spread of germs | Used in surgeries & invasive procedures |
Uses clean techniques (e.g., handwashing) | Uses sterile techniques (e.g., surgical dressing) |
Gloves protect the worker | Sterile gloves protect the patient |
Hand Hygiene
Hand hygiene is the most effective way to prevent infections and stop the spread of harmful germs. It includes hand washing with soap and water and the use of hand rubs (sanitizers).
Hand Washing
Definition:
Hand washing is the process of rubbing hands with soap and water to remove dirt, germs, and microorganisms.
Purpose:
- Removes harmful germs from the hands.
- Prevents infections in patients and healthcare workers.
When to Wash Hands? (as per Gamer and Favero, 1985)
- Before touching patients.
- After patient contact.
- After exposure to body fluids.
- Before invasive procedures (e.g., injections).
- Before and after wound care.
- After handling contaminated equipment.
- Between contacts in high-risk areas.
- After removing gloves.
Hand Washing Procedure
Materials Required:
✅ Running water (preferably warm)
✅ Antimicrobial or regular soap
✅ Clean towel
Steps for Hand Washing:
1️⃣ Wet hands under running water. Keep hands lower than elbows.
2️⃣ Apply soap and rub palms together in circular motions.
3️⃣ Rub the back of each hand with interlaced fingers.
4️⃣ Interlock fingers and rub between them.
5️⃣ Rub the back of fingers against opposite palms.
6️⃣ Rub thumbs in the opposite hand using a rotating motion.
7️⃣ Rub fingertips in circular motions on opposite palms.
8️⃣ Rub wrists, then rinse hands under running water.
9️⃣ Dry hands with a clean towel.
💡 Wash hands for at least 15-20 seconds as recommended by the CDC.
Surgical Hand Washing
Surgical hand washing is a more thorough process to eliminate all microorganisms, including spores and pathogens.
Materials Required:
✅ Antimicrobial soap/antiseptic solution
✅ Running warm water
✅ Nail brush
✅ Sterile towels
Procedure:
1️⃣ Remove jewelry and roll up sleeves.
2️⃣ Check hands for cuts or wounds.
3️⃣ Wet hands and forearms under running water.
4️⃣ Apply antiseptic soap and scrub hands, nails, and arms up to elbows.
5️⃣ Use a nail brush to clean under nails.
6️⃣ Rinse hands under running water, keeping hands above elbows.
7️⃣ Dry hands with a sterile towel, using separate sides for each hand.
8️⃣ Avoid touching anything before wearing sterile gloves.
💡 Key Points:
✔ Always wash from least contaminated (elbows) to most contaminated (hands).
✔ Keep hands above elbows to prevent contamination.
✔ Do not touch the faucet after washing.
Use of Hand Rub (Hand Sanitizer)
💡 When soap and water are unavailable, use an alcohol-based hand rub.
Steps:
1️⃣ Apply a small amount of sanitizer (at least 60% alcohol).
2️⃣ Rub palms together.
3️⃣ Rub the back of hands and between fingers.
4️⃣ Rub thumbs and fingertips.
5️⃣ Continue rubbing until hands are dry.
✔ Quick and effective for killing germs.
❌ Does not remove visible dirt or grease.
COURSES
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